INSTAND e.V.

Reference measurement procedures at INSTAND e.V.

Enzymes:

Alanin Aminotransferase (EC 2.6.1.2)
Aspartate Aminotransferase (EC 2.6.1.1)
Creatine Kinase (EC 2.7.3.2)
gamma-Glutamyltransferase (EC 2.3.2.2)
Lactate Dehydrogenase (EC 1.1.1.27)        

Mesurement of all enzymes with IFCC Reference Measurement Procedures for the Catalytic Activity Concentrations of Enzymes at 37°C

Ions:

Calcium        AAS
Chloride           Coulometry
Lithium            AAS
Magnesium      AAS
Phosphate       Photometry
Potassium       FES
Sodium           FES

Substrates:

Cholesterol (total)
Creatinine
Glucose
Uric Acid
Urea
Triglycerides     Measurement of all substrates with ID/GC-MS

Hormones:

Cortisol
17ß-Estradiol
Progesterone
Testosterone
L-Thyroxine          Measurement of all hormones with ID/GC-MS

Proteins: 

HbA1c                 Measurement with both IFCC Reference
                     measurement procedures LC-MS or HPLC-CZE
total Proteine        modified Biuret reaction

Drugs:

Digitoxin             ID/LC-MS/MS
Digoxin               ID/LC-MS/MS
Theophylline        ID/GC-MS

The reference values for Haemoglobin, Erythrozytes, Leukocytes and pH are determined in cooperation with the german Physikalisch-Technischen Bundesanstalt (PTB)